The importance of a good IVF laboratory
In-Vitro fertilisation is an assisted fertility technique where the egg (ovule) is fertilised outside the woman’s body.
Before beginning in-vitro fertilisation treatment, it is vital to make a diagnosis and to plan a highly personalised strategy, which is adapted to each case. Once all the complementary diagnostic tests have been studied, and the strategy to be followed has been established, ovarian stimulation can begin. This process is medically controlled, and it aims to make the ovaries produce as many optimal-quality eggs as possible.
When the eggs are mature, ovarian puncture begins. This is a simple surgical procedure that requires sedation in the majority of cases. A specialist extracts the mature eggs from the woman’s ovaries. These eggs are then sent to the IVF laboratory to be fertilised using traditional IVF methods or with IVF-ICSI.
In traditional IVF the specialist joins the egg with a previously extracted sample of sperm. Spontaneously and naturally, only one of these sperm will break through the thick skin of the egg to fertilise it.
In an IVF with ICSI process the specialist selects the optimum sperm from the entire extracted sample, and using a microinjection, pierces the thick skin of the egg and deposits the cytoplasm directly. This facilitates the fertilisation process. When realising an ICSI process, the embryo is manipulated more. However, the success rate increases if the specialist has a good grasp of the technique.
The specialist’s control of the technique, the conditions of stability of the IVF laboratory, technical resources or the quality of cultivation methods are important determining factors for in-vitro fertilisation to succeed.
Following successful fertilisation, the embryos are transferred to the woman’s uterus on day 3 or when they are at the blastocyst stage (approximately day 5), depending on the strategy that has been planned for the individual patient. The remaining embryos are vitrificated to potentially be used in the future.
Around 9-10 days after embryo transfer, the woman will take a pregnancy test.